Regulatory
pBar1

Part:BBa_K1829001:Design

Designed by: Nicholas Suen   Group: iGEM15_UCSF   (2015-09-08)


pBar1: Mating factor alpha responsive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We changed nucleotide 210 from thymine to cytosine. This does not change the amino acid Asparagine and is therefore a silent mutation. We also changed nucleotide 285 from cytosine to adenine. This does not change the amino acid valine and is therefore a silent mutation.


Source

pBar1 is found in the genomic sequence of MATa S. cerevisiae.

References